![]() Two autophagy-related genes ( MARCKS and TXNIP) mark high-plasticity GC with poorer prognosis, and autophagy inhibitors induce apoptosis in patient-derived organoids. Transitions occur via the recently described, autophagy-dependent plasticity program, paligenosis. Moreover, we track GC evolution, identifying high-plasticity GC clusters that exhibit a propensity to shift to a high-proliferative phenotype. We also describe the evolution of monocyte-like DCs and regulatory and proliferative T cells following therapy. During GCPM progression, an increase is seen of monocyte-like dendritic cells (DCs) that are pro-angiogenic with reduced antigen-presenting capacity and correlate with poor gastric cancer (GC) prognosis. Here, we characterize single-cell transcriptomes of 191,987 ascites cancer/immune cells from 35 patients with/without gastric cancer peritoneal metastasis (GCPM). The native and therapy-induced ascites ecosystems are not fully understood. ![]() ![]() Peritoneal metastasis is the leading cause of death for gastrointestinal cancers.
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